Journal: bioRxiv

Article Title: IL-1-conferred gene expression pattern in ERα + BCa and AR + PCa cells is intrinsic to ERα − BCa and AR − PCa cells and promotes cell survival

doi: 10.1101/773978

Figure Lengend Snippet: RT-qPCR was performed for select genes for MCF7 and LNCaP cell lines treated with vehicle control or 25 ng/ml IL-1α or IL-1β for 5 days. MDA-MB-231, BT549, PC3 and DU145 cell lines were treated with vehicle control only. (A) RT-qPCR shows that CCL20 , CD68 , IL8 , p62 , SOX9 and Zeb1 are induced by IL-1 in MCF7 and/or LNCaP cell lines and are basally high in MDA-MB-231, BT549, PC3 and/or DU145 cell lines. (B) RT-qPCR shows that CXCR7 and MMP16 , but not CDK2 or PLK1 , are downregulated by IL-1 in MCF7 and/or LNCaP cell lines and are basally high in MDA-MB-231, BT549, PC3 and/or DU145 cell lines. N = 3 biological replicates; error bars, +/−STDEV; p-value, * ≤ 0.05, ** ≤ 0.005, ***≤ 0.0005. mRNA fold change is normalized to MCF7 vehicle control for the BCa cell lines and to LNCaP vehicle control for the PCa cell lines.

Article Snippet: Human recombinant IL-1α (GoldBio, St Louis MO; 1110-01A-100) and IL-1β (GoldBio, St Louis MO; 1110-01B-100) were resuspended in 0.1% bovine serum albumin (BSA, Thermo Fisher Scientific; BP 1600-1) in 1X phosphate buffered saline (PBS).

Techniques: Quantitative RT-PCR

Journal:

Article Title: Leptin directly acts within the hypothalamus to stimulate gonadotropin-releasing hormone secretion in vivo in rats

doi: 10.1113/jphysiol.2002.023895

Figure Lengend Snippet: Plasma concentrations of leptin, oestradiol and progesterone in the six experimental groups examined in this study

Article Snippet: All the MPOA, ME-ARC and AHA groups were perfused with 1.0, 3.0 or 10 ng ml −1 of the recombinant rat leptin (R & D Systems, Inc., Minneapolis, MN, USA) during the period of 14:00-17:20 h. The rat leptin was dissolved in the ACSF immediately before use.

Techniques: Clinical Proteomics

Journal:

Article Title: Leptin directly acts within the hypothalamus to stimulate gonadotropin-releasing hormone secretion in vivo in rats

doi: 10.1113/jphysiol.2002.023895

Figure Lengend Snippet: In this and subsequent figures (2, ​,44 and ​and5):5): (1) data from only the highest concentration of leptin (10 ng ml−1) are shown; (2) the horizontal bar indicates the period during which leptin (▴) or vehicle (ACSF, ○) was infused; (3) the time of perfusate collection in the upper three graphs is shifted 10 min ahead of the actual time of perfusion because the dead space of the pull system (150 μl) corresponds to a 10 min period of perfusion (flow rate, 15 μl min−1); (4) measurements of α-MSH, NPY and GnRH in the perfusates are expressed as point values at the centre of their collection periods; and (5) dotted lines in the graphs for α-MSH, NPY and GnRH indicate the limits of detection for each peptide. Number of rats in each subgroup = 8-10.

Article Snippet: All the MPOA, ME-ARC and AHA groups were perfused with 1.0, 3.0 or 10 ng ml −1 of the recombinant rat leptin (R & D Systems, Inc., Minneapolis, MN, USA) during the period of 14:00-17:20 h. The rat leptin was dissolved in the ACSF immediately before use.

Techniques: Concentration Assay

Journal:

Article Title: Leptin directly acts within the hypothalamus to stimulate gonadotropin-releasing hormone secretion in vivo in rats

doi: 10.1113/jphysiol.2002.023895

Figure Lengend Snippet: Open bars, ACSF (control); bars with horizontal lines, leptin (1.0 ng ml−1); bars with vertical lines, leptin (3.0 ng ml−1); filled bars, leptin (10 ng ml−1).* Statistically significant vs. the ‘before’ values of the respective groups. † Statistically significant vs. the other three groups. ‡ Statistically significant vs. the leptin (1.0 ng ml−1) group.

Article Snippet: All the MPOA, ME-ARC and AHA groups were perfused with 1.0, 3.0 or 10 ng ml −1 of the recombinant rat leptin (R & D Systems, Inc., Minneapolis, MN, USA) during the period of 14:00-17:20 h. The rat leptin was dissolved in the ACSF immediately before use.

Techniques: Control

Journal:

Article Title: Leptin directly acts within the hypothalamus to stimulate gonadotropin-releasing hormone secretion in vivo in rats

doi: 10.1113/jphysiol.2002.023895

Figure Lengend Snippet: Open bars, ACSF (control); bars with horizontal lines, leptin (1.0 ng ml−1); bars with vertical lines, leptin (3.0 ng ml−1); filled bars, leptin (10 ng ml−1). *Statistically significant vs. the ‘before’ values of the respective groups. † Statistically significant vs. the other three groups. ‡ Statistically significant vs. the leptin (1.0 ng ml−1) group. ** Statistically significant vs. the ACSF and leptin (1.0 ng ml−1) groups.

Article Snippet: All the MPOA, ME-ARC and AHA groups were perfused with 1.0, 3.0 or 10 ng ml −1 of the recombinant rat leptin (R & D Systems, Inc., Minneapolis, MN, USA) during the period of 14:00-17:20 h. The rat leptin was dissolved in the ACSF immediately before use.

Techniques: Control